5/1/2023 0 Comments Farsky beta size![]() ![]() In conclusion, our study for the first time explored the BBB pass rate and brain tissue distribution of 5-CQA administered via the tail vein by the UHPLC–MS/MS method and investigated the potential main target area of 5-CQA for neuroprotection, which could provide a certain basis for the treatment of nervous system-related diseases of 5-CQA.Ĭhlorogenic acids are phenolic natural products composed of caffeic and quinic acids found in a variety of plants, fruits, vegetables, and coffee. elegans strain paralysis assay indicated that the neuroprotective effect of 5-CQA is positively correlated with its content in different brain tissues. In addition, 5-CQA can penetrate into the rat brain extensively and is distributed with different intensities in different nuclei. Pharmacokinetic results revealed that 5-CQA can pass through the BBB into the CSF, but the permeability of BBB to 5-CQA (ratio of mean AUC 0-∞ of CSF to plasma) was only approximately 0.29%. Methodological validation results presented a high accuracy (95.69–106.81%) and precision (RSD ≤ 8%), with a lower limit of quantification of 1.0 ng/mL. Chromatographic separation of 5-CQA and glycyrrhetinic acid (GA, used as internal standard) was completed in 0.5 min, and the full run time was maintained at 4.0 min. elegans) strain paralysis assay was used to investigate the neuroprotective effect of 5-CQA in different brain tissues. ![]() In this study, we established a rapid and sensitive UHPLC–MS/MS method for the determination of 5-CQA in rat plasma, cerebrospinal fluid (CSF), and brain tissue to investigate whether it could pass through the blood–brain barrier (BBB) and its distribution in the rat brain, and a Caenorhabditis elegans ( C. However, its pharmacokinetics and distribution in the rat brain remain unclear. Chlorogenic acid (5-CQA) is a phenolic natural product that has been reported to improve neurobehavioral disorders and brain injury. ![]()
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